The Plasmid midiPREP Kit provides a fast, simple, and cost-effective plasmid midiprep method for isolating the plasmid DNA from the cultured bacterial cells. The Plasmid midiPREP Kit is based on the alkaline lysis of bacterial cells, followed by binding DNA onto the glass fiber matrix of the spin column in the presence of high salt. Phenol extraction and ethanol precipitation are not required, and the high-quality plasmid DNA is eluted in a small volume of the Tris buffer (included in each kit) or water (pH is between 7.0 and 8.5). The plasmid DNA purified with the Plasmid midiPREP Kit is suitable for a variety of routine applications, including the restriction enzyme digestion, sequencing, library screening, in vitro translation, transfection of robust cells, ligation, and transformation. The entire procedure can be completed within 40 minutes.
产品特点
此试剂盒提供了一种从培养的细菌中快速、简单分离质粒DNA的方法. 先使细菌细胞壁硷性裂解,后将 DNA 于高盐环境经离心与管柱的玻璃纤维结合. 提取的DNA可使用在限制酶作用,定序,细胞转殖等实验上. 整个提取程序可在 40 分钟内完成.